CD40 antibody (Extracellular Domain) (PE)

Catalog No. ABIN118124

The Rat Monoclonal anti-CD40 antibody has been validated for FACS. It is suitable to detect CD40 in samples from Mouse.

Quick Overview for CD40 antibody (Extracellular Domain) (PE) (ABIN118124)

Target: CD40

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD40 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 01-03-23

Product Details anti-CD40 Antibody (PE)

Binding Specificity: Extracellular Domain

Specificity: This antibody recognises CD40 and stains most mature mouse B cells. T cells are unlabelled.

Purification: Affinity Chromatography on Protein G.

Immunogen: Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled water: SM008R with 1.0 ml. SM008RT with 0.25 mL SM008RX with 5.0 ml

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Gene ID: 21939

NCBI Accession: NP_035741

UniProt: P27512

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints